Dear Richard,
Basic Fuschin absorbs maximally at 544 nm. Note that there are different
grades and formulations: Fluka Chemie AG offers three forms - a powder and
two solutions ("Solution according to Kinyoun" and "Solution acording to
Ziehl-Neelosn"). All three contain phenol and are fomulated for bacterial
staining. Aldrich offers three grades (no phenol) - "certified," 90% and
95%. They also list several references on page 143 of their 2000-2001
Handbook.
There are about 11 references for this dye on PubMed, but only one mentions
use for staining sections. You can access Pubmed/Medline directly from our
home page at http://www.helixresearch.com One of these will likely give an
emission maximum.
Best regards,
Michael Kuhn, President
Helix Research
Fluorescence Chemistry
mkuhn@helixresearch.com
www.helixresearch.com
----- Original Message -----
From: Richard K. Meister <meister.1@osu.edu>
To: cyto-inbox
Sent: Monday, July 23, 2001 10:23 AM
Subject: Basic Fuchsin
>
> Hello, everyone:
>
> I have an ACAS 570 confocal laser scanning cytometer with an argon laser,
> and I have a faculty member who wants to scan slides stained with basic
> fuchsin (rosanilin). I haven't been able to find much information
> regarding the excitation and emission spectra of this dye. I would
> appreciate any information you might have regarding which laser line to
use
> and what wave lengths to use to collect the fluorescence.
>
> Thanks in advance,
> Rick Meister
>
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