Dear Nan, The absorption of YFP is about half at 488nm, so you should see some signal though it may be dim depending on the level of expression; the FL1 channel will be the best choice for detection. You can see the details at: http://nucleus.immunol.washington.edu/Research_facilities/Apps/gfp_overview. html The web site that I built is still there even though I have been an independent consult in cytometry since 1 June of this year. Dave ---------------- David M. Coder, Ph.D. email: dcoder1@qwest.net tel./messages: 206-499-3446 ----- Original Message ----- From: "Nancy Harvey" <nharvey@microbio.umsmed.edu> To: cyto-inbox Sent: Monday, July 23, 2001 9:00 AM Subject: Can EYFP be run on FACScan? > > Hello, > I have an investigator who wants to check samples that have enhanced YFP. The ideal > excitation is 514. Has anyone been able to detect EYFP with the 488 excitation of > a FACScan? > > I could tune the primary laser on the Vantage to 514, but have only 488/10 filters > for FSC and SSC. > > Any thoughts and suggestions are appreciated. > > Nan Harvey > University of MS Medical Center > Jackson, MS 39216 > >
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:57:48 EST