Colleagues, I would like to know if anyone out there has used CMXRos (or more commonly know as Mitotracker Red) from Molecular Probes. More specifically, I am interested in performing multi-parameter stains on cells using CMXRos (emits at 605nm), CFSE (emits at 525nm) and a PE-conjugated antibody (emitting at 575nm). I will be acquiring the data using a BD FACScalibur with filters set at band passes 525nm, 575nm, and 640nm for FL1, FL2 and FL3 respectively. My question is: has anyone had any problem with getting a strong enough signal from CMXRos using a filter set at 640nm keeping in mind that there is only a small overlap of the CMXRos spectral emission with this filter (the spectral emission can be viewed at Molecular Probe's website). Any suggestions would be helpful. Tara McDonald Centenary Institute of Cancer Medicine and Cell Biology Locked Bag No. 6 NEWTOWN, NSW, 2042 Australia
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