[Fwd: [Fwd: Converting data from Mac]]
From: Ana Salas (ana@lupo.medicina.uniovi.es)
Date: Tue Jul 17 2001 - 07:31:49 EST
attached mail follows:
Hola Ana.
Tal vez esto llegue un poco tarde pero el 13 ya estaba de vacances.
> Subject: Converting data from Mac
> Date: Fri, 13 Jul 2001 14:10:00 +0200
> From: Ellen.Freed@astrazeneca.com
> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
>
> Hi All
>
> We're considering buying a FACScalibur from BD, but are concerned that BD is
> all in MAC format and all our computers are PC's . I was told by someone at
> BD that this shouldn't be a problem, because everything could be easily
> converted if need be - but I've heard of others who've had problems with this.
>
> I have been working with a FACScalibur conected to a Mac. The data files
> generated by this system are compatible with other flowcytometer PC's
> software, including cell cycle analysis. There was no need for data
> conversion. The only difference is that data files generated by PC have an
> extension .fcs and the ones the generated under Mac have no extension or
> something like .001 that is not a real file extension. However, files
> generated under Mac with a file name as RQ.001 are directly read by Modfit
> under PC.
>
> We'll need to do cell cycle analysis using Modfit or Multicycle, which we'll
> probably want to have on a couple of remote computers which are PC's. Any
> experience with this problem?
I have carried cell cycle analysis using Modfit both in Mac and PC's versions.
>
> Also, I know that CellQuest is also only in MAC format. What does CellQuest
> offer that we couldn't do with the above-mentioned cell cycle programs?
>
Cell Quest is the software that adquires the data directely from the
flowcytometer and where you can adjust the receptor channels (intensity,
compensation, threshold), the data that you want to store in the data files, the
number of cells to analyse, etc.
Modfit carries out the model analysis to determine cell cycle populations from
the files generated by Cell Quest. I have not used Multicycle.
I have been working with a FACScalibur in Bergen (Norway) a few months ago. Data
files were generated by Cell Quest (under Mac). I sent several of those files to
the technician of the University of Oviedo (that works with PC) to carry out the
cell cycle analysis and she couldn´t read them. In the begining, we thought that
it was a matter of Mac-PC compatibility, but turned out to be a problem of data
transference through email, that for some reason the files were somehow altered.
All the data files generated by the FACScalibur that I worked with in Bergen
(conected to a Mac) are read for cell cycle analysis by Modfit under a PC.
>
> Thanks to anyone who can help.
>
> Ellen Freed
> Scientist
> Cancer Bioscience
> AstraZeneca R & D Boston
--
Rafael González-Quirós
Universidad de Oviedo
Dpt. B.O.S. (Área Ecología)
c/ Catedrático Rodrigo Uría s/n
Oviedo 33071
Spain
Tfn: +34-985104831
Fax: +34-985104866
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