I have had an enquiry from someone in the brewing industry regarding flow cytometry methods for yeasts. They are specifically interested in Cell cycle analysis with PI, Viability with PI/ Fluorescein diacetate, Intracellular pH, Neutral lipids with Nile Red?, Glycogen with acriflavin, Mitochondria with Rhodamine 123, flocculation with ConA-FITC lectin. I have had no experience with any of these areas and was hoping that someone out there may be able to point me in the right direction. Any tips or references would be much appreciated. Thanks, Jan Hesketh Molecular Medicine University of Auckland phone 09 373 7599 ex 4492 fax 09 373 7492
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