Knowles (Neoplastic Hematopathology) has a nice discussion on CD45 expression and B-cells. Briefly summarizing: CD45 expression appears to occur very early in B-cell ontogeny, apparently prior to immunoglobulin rearrangement, and continues throughout B-cell differentiation until about the preplasma cell stage. Most immature and mature B cells are recognized by anti-CD45, anti-CD45RA, and anti-CD45RB but not any anti-CD45RO. These include primary follicle and mantle zone B-cells. Transformed B cells and preplasma cells tend to lose CD45RA and may be reactive with anti-CD45RO MAbs. Plasma cells are not recognizable with anti-CD45RA or anti-CD45RO MAbs. J Myers -----Original Message----- From: Frederic Preffer [mailto:preffer@helix.mgh.harvard.edu] Sent: Monday, June 25, 2001 10:34 AM To: cyto-inbox Subject: Re: CD45RO Most circulating B cells expressing sIg are CD45RA+. I am unaware of this being associated with the cells state of activation. F Preffer At 10:50 AM 6/22/01 -0700, Otten, Gillis wrote: > >I know about CD45RA/RO as markers for human T cell differentiation, but has >anyone heard of using CD45RO or CD45RA as human B cell differentiation or >activation markers? Any insights would be appreciated. > >Gib Otten >Chiron Corp >Emeryville, CA >** ** ATTENTION! This message contains information (including any appended information, >such as copied or forwarded messages and attachments, and any message to which this is >appended) which may be confidential and/or privileged. Unless you are the addressee (or >authorized to receive for the addressee), you may not use, copy or disclose to anyone the >message or any information contained in the message. If you have received the message >in error, please advise the sender by reply e-mail, and delete the message. Thank you >for your assistance. ** ** > Frederic I. Preffer Department of Pathology Charlestown Navy Yard- 7140 149 13th Street Massachusetts General Hospital -East Charlestown, MA 02129 voice [617] 726-7481 fax [617] 724-3164
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