Please forgive these basic questions from a new flow person regarding the best way to store lymph nodes for Leukemia/Lymphoma evaluation when the node comes in late in the day and must be kept for the following morning. Should I place the intact node in RPMI or should I go ahead and collect the cells from the node and place them in RPMI? Should I then place the node and/or cells in the refrigerator or leave out at room temperature? Thank you.
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