Hi colleges: We have a patient, a woman of 35 years old with 85,500 WBC/ul reported as 97% blasts. Flow cytometry showed in SSC Vs CD45 most of the cells with low complexity and all of them is an smear in CD45 between negative, low and intermediate positive cells never reach the mature CD45 high position. These population is rather similar in markers CD19+, CD22+, CD20-, CD10-, CD34 -, sIg- and expressed CD5+ intermediate.They are 90% of the BM. Patologist were not happy with CD20 and repeatly was negative using adequate controls for the anti CD20 mAbs. T and myelod markers are negatives.We are wating for the biopsy. Suggestions ? JOHN MARIO GONZALEZ M.D., Ph.D. Facultad de Ciencias Pontificia Universidad Javeriana Phone 00 571 2850503 Fax 00 571 3208320 Ext 4085
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