4-colour FACScalibur Q

From: Joseph Webster (J.Webster@centenary.usyd.edu.AU)
Date: Mon Jun 18 2001 - 22:41:59 EST 

A question to anyone using 4-colour FACScalibur - what mix
of fluorochromes are you using successfully?

In particular, what dyes are you using in FL3 that do not
spill over into FL4?
I'm guessing PerCP and PE/TxRED, what else?

I guess the other side of that question is significant too,
what dyes ARE giving channel spillover problems?

TIA, Joseph.
--
Joseph Webster, Flow Cytometry Facility
Centenary Institute, Sydney AUSTRALIA.

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