Hello, this is probably one of those "stupid" questions that anyone in posession of a flow cytometer should be ashamed to ask: We would like to determine the size distribution of polymer/lipid micelles which we can fluorescently stain with nile red. We have fluorescent beads from Molecular Probes that are about 0.02um and about 1um in diameter to use as size standards. We suspect the micelles to be somewhere around 0.5 um in diamater. How do we go about translating the FSC readings to sub-microns? Is it a linear parameter? Thank you, Snezna
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