I have a client who is interested in looking at the poidy level of her megacaryocyte cell line. The first time she came to the lab she brought her samples and had used Vindelov's. Her histograms looked just like cell cycle histograms. Not surprising as Vindelove's is used for cell cycle. She admitted that when she had looked at ploidy levels years ago she did not use Vindelov's, but did use a PI solution. Can anyone offer what PI protocol she should be using for poidy levels, and if I should be approaching the set up of the Flow Cytometer in a different manner than cell cycle work. I have a client who looks at the ploidy level of plants, but I know that they have to digest the plant material first so I don't think their protocol would be appropriate for a cell line. All input will be greatly appreciated, and as usual, Thanks in advance, Joan Kalnitsky Flow Cytometry Lab Supervisor VMRCVM (540) 231-4115 FAX 540-231-7367 jkalnits@vt.edu "It is better to serve than to receive." B. Borg
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:57:44 EST