Hi all, Some time ago I asked you some questions about CFSE staining of human HSC. You were very helpful and I got some very useful tips. One was to keep the cells overnight after staining to enable quenching of the dye. Now, does anybody have a reference where this was actually tested and proven. I have searched already in PubMed and Medline, without success. I would be very grateful if you could help me on this. Kind regards, Tessa Kerre
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