To: cyto-inbox I have a request from a person for flow cytometric help in identifying the number of chromosomes replicating DNA in a bacterium. He has altered proteins he/they think are involved in bacterial chromosome replication so they want to compare the parent strain to the altered strain. He wants to be able to establish significantly altered chromsomal replication patters having 3 or more replication forks. Any body out there in the US doing this? How about world wide? Has anybody out there tried to do this on a jet in air FACS unit? (You may laugh now. If by some miracle somebody did get even close to making this work--drop me a line will you?) Does anybody have a microscope based FACS unit that will do this? Partec or other variety? Any useful comment from experience will be greatly appreciated. Louis King Louis King, Ph.D. Michigan State University Flow Cytometry Center Department of Biochemistry and Molecular Biology, Rm 419 Michigan State University East Lansing, MI 48824 Phone: 517-355-1536 FAX: 517-353-9334 E-Mail: kingL@pilot.msu.edu
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