At 03:58 PM 5/22/01 -0400, you wrote: > >FLOWers > > I am in need of a protocol to fix RBC's from mammalian species >(other then human) > > I would like to stabilize the antigens on the surface and then stain >post fixation. > >Does anyone have any ideas > >Thank you for your time and attention > >Philip Barren > > Greetings Philip, I needed to do some surface/intracellular staining on RBCs a few months ago. I tried the method of Davis et al from the 1998 transfusion paper 'Detection of fetal red cells in fetomaternal hemorrhage using anti-hemoglobin F monoclonal antibody by flow cytometry Transfusion, 38:749-756' -Seemed to work ok for surface/intracellular staining Best regards Arnold _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/ Arnold Richard Pizzey Department of Haematology Royal Free and University College London Medical School 98 Chenies Mews London WC1E 6HX U.K voice: +44 (0)20-7679-6234 Fax: +44 (0)20-7679-6222 email: a.pizzey@ucl.ac.uk _/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/_/
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