Hello Flowers, Here is a question I am passing on from a colleague who is working on primary cultures of cerebellar granule cells. These cells appear to be very adherent and he is having great trouble keeping them apart long enough to sort them on a flow cytometer. He has tried trypsin and DNase and EDTA at various concentrations ---and the cells still become unmanageably viscous before they can be sorted. Does anyone have any ideas? Alice Alice L. Givan, Englert Cell Analysis Laboratory of the Norris Cotton Cancer Center Dartmouth Medical School Lebanon, New Hampshire NH 03756 tel 603-650-7661 fax 603-650-6130 givan@dartmouth.edu
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