Dartmouth Flow Course

From: Alice L. Givan (Alice.L.Givan@dartmouth.edu)
Date: Tue May 22 2001 - 08:43:17 EST


Hello Flowers,
This morning I received a note from someone who said he had only just become aware of
the flow course being given at Dartmouth in July.  The Course is filling up rapidly,
but at the present time we do still have a few places left -- so please pass this
announcement along to anyone who might be interested and who is not on the Purdue
mailing list.  Thanks.

The Course runs from the 15th - 20th of July (with an optional introductory workshop
on the afternoon of July 14th).

The website ---- giving the course brochure with application form --- can be found at

http://www.hitchcock.org/pages/ceb/brochures/flow.htm

This web site is a full copy of the brochure,  but you can also get a paper brochure
by contacting
karen.griswold@dartmouth.edu
and sending her your mailing address.

As far as other information about the course:

In order to have more time for lectures on specific advanced topics during the Course
itself, we are giving an optional tutorial workshop on introductory flow theory on
Saturday afternoon, July 14th.   This session will include informal lectures and
discussion on how a flow cytometer works,  choice of fluorochromes, compensation
for spectral crossover, staining procedures, and basic methods for data analysis.
There will be plenty of time for questions.

The Course itself starts in the morning on Sunday, July 15th, with a day of lectures,
a banquet, and a keynote address by Zbigniew Darzynkiewicz on apoptosis.  The rest of
the Course, (Monday to Friday at noon) will have a lecture first thing in the morning
followed by a 3-4 hour laboratory module.  After lunch, there will be another lecture,
followed by another laboratory module.  The lectures will be on subjects related to the
laboratory modules,  but also on subjects that point to additional topics of interest.
Evenings will have panel discussions (for example,  on quality control issues and on
the use of flow data for clinical diagnosis) and also an open "ask the experts" session.

The laboratory modules for the 2001 Course are listed below. In addition to the  common
clinical applications, we will have several  "frontier" labs with Nadège Bercovici
teaching about tetramer staining, Kathy Muirhead teaching about tracking cells for
cell division, Frank Mandy including multiplex bead assays in his HIV lab, and Doug
Taatjes running a module on the laser scanning cytometer.

1) Carleton and Sigrid Stewart: multicolor leukemia and lymphoma phenotyping
2a) Kathy Muirhead: cell  tracking
2b) Nadège Bercovici: tetramer staining
3a) Mike Keeney: CD34 stem cells in rare event analysis
3b) Doug Taatjes: laser scanning cytometry
4a) Kenneth Ault: platelets
4b) Bruce Davis: rare fetal cells in the maternal circulation
5a) Bruce Bagwell: data analysis for DNA/cell cycles
5b) Paul Wallace, Alice Givan: staining cells for DNA content
6) Jake Jacobberger: multiparameter staining and analysis for intracellular proteins,
surface proteins, DNA
7) Frank Mandy and Michele Bergeron: HIV/AIDS, including CD4/CD8 quality control and
multiplex bead assays for soluble analytes.

We will limit the Course to about 45-50 participants. In the past they have come from
many countries. They have been  from universities, hospitals, and biotech companies
---- in short a wide range of different types of people.  The participants rotate in
groups of about 8 through all the lab modules

Let me know if I can give you any further information.  You can phone me directly
(603-650-7661) if you have any questions about the content of the Course.  Karen Griswold
(605-653-1522) can answer any administrative questions (about registration, housing,
travel, etc.).

Alice

Alice L. Givan, Ph.D. ( with Paul K. Wallace, Ph.D.), Course Co-Director
and
Director, Englert Cell Analysis Laboratory
of the Norris Cotton Cancer Center
Dartmouth Medical School
Lebanon, New Hampshire NH 03756
tel 603-650-7661
fax 603-650-6130
givan@dartmouth.edu



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