Larry: We have the original Enterprise laser on our confocal - it was installed in 1994 and has been operating nonstop eversince. We run it at a fixed power of 60 mw which draws about 23 amps. It permanently runs with the 488/UV mode. I don't know exactly how many hours are on it , but its used essentially every work day for the past 6 plus years and so far it has not failed......I guess it has to eventually --- when I purchased it I was concerned as there had been some problems with these lasers...Coherent assured me they had redesigned the Enterprise and that it was a stable long lasting platform - so far I have to agree..... The confocal system has a complex excitation filter and ND system to regulate each line all of which are electronically controlled....maybe in about 10 years time the flow people will have a system almost as easy to use and modify as the confocal people had 6 -7 years ago...... Paul Robinson Date sent: Thu, 17 May 2001 13:32:46 -0400 To: cyto-inbox From: Larry Arnold <lwarma@med.unc.edu> Subject: Enterprise laser experiences I would like to request info from people who use/know the Enterprise laser from Coherent. I have always been uneasy with using this laser for simultaneous 488 and UV primarily because the laser is light regulated on only one of the lines at a time. Thus, I worried that over time the unregulated line might change and affect experimental results. Also for the unregulated line you just get whatever you want based on what power output you want from the regulated line (is this correct?). Short of using filters to reduce the power if needed I assume this is the situation. However, I have no direct experience and am now considering a system with that laser. What has the experience been? Is this a real worry but not actually realized? What is the lifetime ratio of the 488/UV lines? I appreciate your advice and thanks in advance. Larry Larry W. Arnold, Ph.D. Res. Assoc. Prof. Director, Flow Cytometry Facility Department of Microbiology and Immunology Lineberger Comprehensive Cancer Center CB# 7290 University of North Carolina at Chapel Hill Chapel Hill, NC 27599 Phone: 919-966-1530 FAX: 919-962-8103 J.Paul Robinson, PhD PH:(765)4940757 Professor of Immunopharmacology Professor of Biomedical Engineering Purdue University FAX:(765)4940517 EMAIL:jpr@flowcyt.cyto.purdue.edu WEB: http://www.cyto.purdue.edu
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