Yes, about 1% of individuals have a mutation which prevents the splicing of the CD45 RNA into the RO form; thus, all the cells express CD45RA. There's actually a publication on this in the literature, about 10 years ago, but I can't for the life of me remember where or what. In a clinical trial we ran some years ago, where we quantitated the relative expression of naive and memory T cells, we put an exclusion criteria for entry into the clinical trial to eliminate individuals with this genetic variant (I wouldn't call it a "defect"--they have no apparent immunological or other abnormalities). We excluded a few out of the 800 individuals we screened. It's definitely hereditary; one of our lab techs had this phenotype, as did his mother and sister. mr At 1:02 PM -0400 5/17/01, Aki Hoji wrote: >Dear all, > >Does anybody know if there is a hereditary defect in CD45RO expression (due >to splicing error ?) in human ? I have one individual whose PBMC are >negative for CD45RO, and they are either CD45RA dim and bright. > > >Aki Hoji >University of Pittsburgh >Department of Infectious Diseases and Microbiology >Rm522 Parran Hall, GSPH-IDM >130 Desoto Street, Pittsburgh PA 15261 >tel: (412) 624-3072/0776 >fax: (412) 624-4953
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