I have an investigator who wants to measure levels of Zn++ in his cell lines. Has anyone had any experience with Fura-2 that could give us some pointers? We are planning to excite this dye with a multi-line UV and collect the emission at 510. The literature that we have found just uses spectrophotometry to measure this metal. Can we do this? Thanks, Kathy -- -------------------------------------------------------------- Kathy Schell Supervisor, UWCCC Flow Cytometry Facility 600 Highland Ave. K4/535 Madison, WI 53792 Voice: 608-263-0313 e-mail: kschell@facstaff.wisc.edu
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