Obviously the UK and US definitions of Class 1, 2 and 3 are different - and reversed. Robin Barclay ----- Original Message ----- From: "Simon Monard" <smonard@trudeauinstitute.org> To: cyto-inbox Sent: Thursday, May 17, 2001 2:17 PM Subject: Re: Primary Human cells On the contrary! A Class I biological safety cabinet is a negative pressure ventilated cabinet usually run with a face velocity of 75fpm or more. The cabinet is exausted either through a HEPA filter or to the outside. So, a Class one cabinet would protect the operator but would NOT help keep the sample aseptic. A Class II biological safety cabinet on the other hand has HEPA filtered downward vertical laminar airflow for sample protection and HEPA filtered exaust for environmental protection. I refer you to the CDC Biosafety in Microbiological and Biomedical Laboratories handbook for such definitioins. Simon Monard FACS Lab Manager Trudeau Institute Saranac Lake NY12983 Ph 518 891 3080 X352 >>> "Robin Barclay" <robin.barclay@ed.ac.uk> - 5/16/2001 5:51 AM >>> Surely Class 2 would be a minimum requirement for operator safety? Class 1 might keep the sample aseptic, but wouldn't protect the operator. Robin Barclay ----- Original Message ----- From: "Simon Monard" <smonard@trudeauinstitute.org> To: cyto-inbox Sent: Tuesday, May 15, 2001 2:59 PM Subject: Re: Primary Human cells > > I would not sort primary human or primate material without some sort of aerosol > containment, wearing a mask for more than an hour or two is very uncomfortable. I have > a custom Class one type hood on my Vanatge > > > > Simon Monard > FACS Lab Manager > Trudeau Institute > Saranac Lake > NY12983 > > Ph 518 891 3080 X352 >
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:57:38 EST