> ---------- > From: Shields, Dottie > Sent: Monday, 14 May 2001 23:14 > To: Cytometry Mailing List > Subject: Bone Marrow Core Biopsy > > Dottie, > > I use a mechanical tissue dissaggregator (Medimachine) for trephine > specimens and other biopsies. There are several suppliers- Becton > Dickinson is one. Works well for all specimens except very fatty ones. > > Greg Hodge PhD. > > -------------------------------------------------------------------------- > ------------- > > I have tried to flush cells from a core bx. of leukemia patients who have > a > packed marrow. I've used a TB syringe to flush PBS through the core and > even tried crushing the core. I've not had much success in getting enough > cells for flow, but when the core is processed by histology there are > still > a lot of cells there. Does anyone have s better method or releasing the > cells from the biopsy? > > Thanks, > Dottie Shields, MT(ASCP), > Flow Cytometry Supervisor > > Fox Chase Cancer Center > 7701 Burholme Ave. > Philadelphia, PA 19111 > Phone: (215) 728-2492 > Fax : (215) 728-2899 > E-mail: d_shields@fccc.edu >
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