RE: Bone Marrow Core Biopsy

From: Hodge, Greg (HAEM) (hodgeg@mail.wch.sa.gov.au)
Date: Mon May 14 2001 - 22:36:05 EST


> ----------
> From:		Shields, Dottie
> Sent:		Monday, 14 May 2001 23:14
> To:	Cytometry Mailing List
> Subject:	Bone Marrow Core Biopsy
>
> Dottie,
>
> I use a mechanical tissue dissaggregator (Medimachine) for trephine
> specimens and other biopsies. There are several suppliers- Becton
> Dickinson is one. Works well for all specimens except very fatty ones.
>
> Greg Hodge PhD.
>
> --------------------------------------------------------------------------
> -------------
>
> I have tried to flush cells from a core bx. of leukemia patients who have
> a
> packed marrow.  I've used a TB syringe to flush  PBS through the core and
> even tried crushing the core.  I've not had much success in getting enough
> cells for flow, but when the core is processed by histology there are
> still
> a lot of cells there.  Does anyone have s better method or releasing the
> cells from the biopsy?
>
> Thanks,
> Dottie Shields, MT(ASCP),
> Flow Cytometry Supervisor
>
> Fox Chase Cancer Center
> 7701 Burholme Ave.
> Philadelphia, PA 19111
> Phone: (215) 728-2492
> Fax :    (215) 728-2899
> E-mail: d_shields@fccc.edu
>



This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:57:38 EST