Dear group, I have a question regarding the following statements, in context of immunophenotyping CD4/8/19/3/16+56 in HIV+ patients: 1. If you run 3 or 4 color flow, isotype control is not required 2. If you run 3 or 4 color flow, and define lymphs on SSCvs.CD45, purity is assumed 100% my problem with 1 is that every once in a while I'll see a trail of cells in the isotype tube (usually IgG1Fitc/IgG2aPE, BD) which does not go away with 3 colors (as in .. fsc/ssc gate vs. ssc/CD45 gate) my problem with 2 is if I run CD45Fitc/CD14PE/CD45PE-Cy5, gate on ssc/CD45PE-Cy5, and look at the scatter plot of CD45Fitc/CD14PE, the CD45Fitc+/CD14PE- cells will be almost always less than 100% (usually greater than 97%) These statements come from our set of guidelines for a inter-lab quality assessment. What does everyone else think about this? ===== `---------------------------------------------` | Maciej S. Simm | 525 E 68th Street | | Research Technician | Room N-805 | | Cornell Medical Center | Tel. 212.746.3428 | `---------------------------------------------` |www.cd4cd8.com | 917-734-6280 | AIM - XsimmX | `---------------------------------------------` __________________________________________________ Do You Yahoo!? Yahoo! Auctions - buy the things you want at great prices http://auctions.yahoo.com/
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