Wayne, What wavelength does your UV laser operate at? I'm about to try Alexa 350 on an LSR which has a 325nm UV laser and it has been intimated to me that it may not work very well. And I won't have the option of adjusting output. I for one would be interested in your results. David McFarland GlaxoSmithKline ---------------------- Forwarded by David C McFarland/DEV/PHRD/SB_PLC on 05/09/2001 07:40 --------------------------- wayne.turnbull@kcl.ac.uk on 07-May-2001 18:34 To: cyto-inbox cc: Subject: Power requirements for Alexa350 excitation Just a very quick question - can someone with experience of Alex350 use in flow tell me how much laser power is usually needed to get a decent signal? I have a neutravidin Alexa350 that stubbornly refuses to show itself and I can't go over 50 mW or else I'll start leaving burn marks on my UV line LP splitter. Cheers Wayne W Turnbull Flow Cytometry Services Immunobiology Guys Hospital London
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