Hi, you may get help from the following publications: Ciudad et el Immunophenotypic analysis of CD19+ precursors in normal human adult bone marrow: implications for minimal residual disease detection. Haematologica. 1998 Dec;83(12):1069-75. Lucio et al. Flow cytometric analysis of normal B cell differentiation: a frame of reference for the detection of minimal residual disease in precursor-B-ALL. Leukemia. 1999 Mar;13(3):419-27 Ciudad et al. Detection of abnormalities in B-cell differentiation pattern is a useful tool to predict relapse in precursor-B-ALL. Br J Haematol. 1999 Mar;104(4):695-705. The articles refer to how to determine % of various subpopulations using live gate analysis. We use that method and express MRD as percentage of total BM cells. Comparison with morphology shows that he morphological counting of blasts in the smears (still used in some protocols) have obviously little to do with the real % of leukemic cells in the BM after therapy. Using that method you can also see if the maturation of B-precursors in the BM follows normal pattern. Kind regards Anna Anna Porwit Hematopathology Lab. Department of Pathology, Radiumhemmet Karolinska Hospital, 171 76 Stockholm, Sweden Anja.Porwit@ks.se tel.:+46-851774518 fax.:+46-851775843
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