Hello again, 3 weeks ago, I tried to get some information about the analysis of intracellular interleukins in monocytes. A lot of people asked me to forward the answers to them, but until now I got only one answer (thanks to Joost). Therefore I want to repeat my questions again and forward my original mail. We would be happy about every help. Andreas ------- Forwarded message follows ------- From: Self <andreas.simm@medizin.uni-halle.de> To: cyto-inbox Subject: intracellular interleukins in monocytes? IL-18 antibody? Date sent: Thu, 12 Apr 2001 16:27:38 +0200 Hello everybody, we want to analyse the expression of interleukins in monocytes. During the first analysis, a lot of questions appeared. Therefore, I asked some people and got no really clear answers. Hopefully, somebody can help me. 1) We want to stimulate whole blood for up to 24 hours and analyse the monocytes afterwards. In which sort of tubes should we keep the blood or is it possible to block the attachment of the monocytes to the tube material? 2) If the monocytes are attached to the wall, is it possible to include them in an analysis or are all of them stimulated by the attachment per se? 3) We see that one have to predilute the blood in RPMI or AIM-V medium before one stimulate the cells. Which medium is the better one and why do one have to dilute it? 4) Which heparin blood is the better one, Li-heparin or Na-heparin? I see the question from yesterday and hope to get the answer as well. 5) Do anybody know a source of IL-18 antibodies for intracelluilar staining? Thanks for your help in advance Andreas Simm ------- End of forwarded message ------- Andreas Simm Klinik fuer Herz- und Thoraxchirurgie Universitaet Halle Ernst-Grube-Str. 40 06120 Halle Tel.: +49 (0) 345 557 2647 FAX: +49 (0) 345 557 2782
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