Dear all, Does anyone have a protocol to fix human parasitized RBCs without losing any of them? I am attempting to surface stain RBCs with various antibodies, some pure, some not. I would like a protocol to fix them to stain them later or stain first and read them later. What alterations in flow readings occur as a result of your protocol? I have just come across the 4% glucose addition to a 10% formaldehyde preparation. However, this seems very harsh and they did not stain for surface receptors. best regards, Bob Bowden
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