Greetings All, I am always happy to learn new things, (thanks Yuri) and so I am asking for input on the following: For years I have been adjusting the beam *focus* on our sorter with help from a pulse width signal, in this case, FSC width. The idea is that the smallest width represents the smallest beam spot. *I use glut.-fixed chicken red blood cells on a FACStar Plus. (100u nozzle) with an elliptical spot. *488nM laser at 60mW. *The CV's I get on PI-stained CTN are 3% at 256 channel resolution, which I believe for this type of instrument is quite good. Someone has my 2nd edition Shapiro tome and the cytomut treatise is therefore not available to me now. Is my reasoning on target? Constructive comments please. Cheers, Sam Sam Witherspoon sw11527@gsk.com Tel. 919-483-3078 GlaxoSmithKline R&D Page 919-857-7768 5 Moore Dr. Fax 919-483-0585 RTP, NC 27709
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