Hi Jane, we use SYBR-14 for staining DNA on live cells. See our chapter in Current Protocols in Cytometry 11-14 January 2001 and our papers in cytometry 40:353-360 and BMC pharmacology (2001) 1:1. This recent paper can be downloaded free of charge from http://www.biomedcentral.com/1471-2210/1/1 Kind regards Rafael -- Rafael Nunez M.D. Associate Laboratory Member-Immunology Program Manager Flow Cytometry Core Facility Memorial Sloan-Kettering Cancer Center Box 98, 1275 York Avenue New York, NY 10021 Telephone (212) 639-6392 Fax (917) 432-2333 E-mail: nunezr@mskcc.org
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