PMA differentiated THP-1: harvest and cytometric analysis.

From: Witherspoon, Sam (sw11527@glaxowellcome.com)
Date: Fri Apr 20 2001 - 09:58:44 EST


Greetings Flowers,

	I have been engaged to examine surface markers on PMA- treated THP-1
cells by flow.	I expect the cell-removal process to be difficult, and while
there are references to be found which include such cytometric data, the
details on cell harvest have been sketchy.
	I would appreciate any tips from those with experience in this area.
I will be happy  to consolidate and post replies to the group.

	Thanks in Advance

	Cheers,
		Sam
Sam Witherspoon
sw11527@gsk.com
				 Tel. 919-483-3078
GlaxoSmithKline R&D		 Page 919-857-7768
5 Moore Dr.			 Fax  919-483-0585
RTP, NC  27709



This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:57:34 EST