Rick, An antibody to NK1.1 isn't going to work on Balb/c mice-look at the mouse alloantigen chart on page 126-127 of the B-D Pharmingen catalog. Pharmingen has several pan-NK cell Abs that should do a better job. I'll be interested to hear whether anyone offers an explanation for your loss of CD3-PerCP signal! ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ Mandy A. Cromwell, Ph.D. Research Fellow Harvard Medical School Department of Immunology New England Regional Primate Research Center One Pine Hill Drive Southborough, MA 01772 (508)624-8022
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