Hi Tom,
You could look at Anomeric's reagents, I haven't tried them and don't know
the relative specificity of "Fungalase" compared to "Bacterase", but you
never know...
Ray
Ray Hicks
________________________________________________________________________
|University of Cambridge |Tel 01223 330149 |
|Department of Medicine |Fax 01223 336846 |
|Level 5, Addenbrookes Hospital |e-mail <rh208@cus.cam.ac.uk> |
|Hills Road Cambridge |Web http://facsmac.med.cam.ac.uk |
|CB2 | http://www.med.cam.ac.uk |
|UK |ftp server ftp://131.111.80.78 |
|_________________________________|_____________________________________|
> From: "R. Thomas Leonard" <tleonard@mail.wistar.upenn.edu>
> Date: Tue, 10 Apr 2001 15:28:03 -0400
> To: Cytometry Mailing List <cytometry@flowcyt.cyto.purdue.edu>
> Subject: Any Fungus Among Us?
>
>
> Question from a novice here:
>
> Is anyone aware of a fluorescent stain, or combination of stains that will
> allow me to distinguish fungi from mixed population of fungi and bacteria?
> For that matter, has anyone explored the detection/enumeration of fungi (in
> general, or genus/species specific) using FCM?
>
> I'm interested in counting fungi in samples, but the stains I'm familiar
> with (e.g. calcofluor white) would not likely differentiate fungi from
> bacteria, cellulose, and carbohydrate-containing background debris.
>
> Thanks,Tom
>
>
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:57:33 EST