Hi everybody, With confluent I assume you mean the cells are at their maximum density on a plate: (which I believe doesn't mean they will not continue to divide) I haven't heard any reports here about confluence prior to sorting affecting the condition of post sorted cells; however this might depend on the particular cell line. In a seminar a few months back an investigator stated that a mouse myeloblast cell line drops dead after a certain amount of cell divisions. (No personal experience there). If true then perhaps sorting when non-confluent would be better for that cell line and all involved. I would think that the more confluent a plate is the more toxins produced which in turn influence the health of the cell ? Regards Ann At 11:47 01.04.01 -0700, you wrote: > >Hi- > >A grad student here asked me the following question. I couldn't come >up with any hard evidence from my experience so I would like to ask >for any feedback that others may have. Thanks. > >Marty Bigos >Gladstone Flow Core > >"I was wondering what you knew about the affect of sorting a cell >line on the health of the cells. Is it thought that you get >healthier cells out post-sort if the cells start from a confluent or >not yet confluent plate? Does it matter?" > >
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