PI/Annexin analysis of Mouse Epithelial cells

From: PAUL HALLBERG (Paul.Hallberg@mail.tju.edu)
Date: Mon Mar 26 2001 - 09:51:17 EST

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Hi FLOWers,

I would be grateful for any helpful tips regarding PI/Annexin analysis of
Mouse Epithelial cells. These adherent cells were removed using 0.5%
trypsin. My unstained cells and PI/Annexin stained cells give a fluorescent
profile difficult to interprete, meaning a "smear" or not a clear
distinction between single positives vs. double positives.

Thanks in advance,

Paul L. Hallberg
CORE Flow Cytometry Facility
Kimmel Cancer Center/Thomas Jefferson University

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