Hi Sandeep,
Your problem may be in the FL2 amplifiers. A quick way to troubleshoot
the Log amp circuit in the BD Benchtop is to swap the amplifier circuit
boards. The SSC/FL2 amp PCB in slot number 3, and the FL1/FL3 amp PCB in
slot 4, are identical, with the only difference in the jumper setting. The
jumper should be blue in color, is labeled JMP 1, and is located in the
approximate center of the circuit board. Before swapping the boards make
note of the jumper setting. I believe JMP 1 setting for the SSC/FL2 PCB are
pins 2 and 3 jumpered together, and FL1/FL3 PCB pins 1 and 2 are jumpered
together. After swapping the boards, run Autocomp again to see if your
problem went away. If the problem goes away after you swap the boards, you
probably need your FL2 Log amp calibrated.
You may also want to run you beads with Autocomp settings in Lysys,
measuring the difference between the mean of the positive and negative
peaks. Check the minimum separation requirement of Autocomp and see if they
pass that way.
Hope this helps.
Regards,
Raymond Lannigan
Tritech Inc.
2014 Renard Ct. Suite I
Annapolis, MD 21401
1-410-266-1522
1-800-886-7004
www.tritechinc.com
-----Original Message-----
From: sandeep ramalingam <sandeeprk@yahoo.com>
To: cyto-inbox
Date: Thursday, March 22, 2001 4:02 PM
Subject: Apologies for wrong info about SSC sensitivity (actually FL2)
>
>Hello again,
>
>I am very sorry to have wasted your time giving you
>wrong information about the problem with the SSC
>sensitivity. Actually I meant a problem with FL2
>sensitivity. I have corrected the question and once
>again apologise for the mistake.
>
>To answer a question that has already been raised, the
>flow cell is new (about 8 months old) and I try to
>maintain as clean a system as possible.
>
>The modified Q is below.
>"We have an old FACScan linked to a HP platform. It
>has been OK until recently. When I caliberate the
>machine (with Autocomp)FL2 sensitivity fails as the
>lower limit is not detected. I have tried
>three lots of Calibrite beads (all within expiry, but
>all three were not uniform suspensions), but the
>problem remains. The BD service engineer also tried
>swapping the FL2 and FL3 PMT's unsuccessfully (is it
>allowed?). He has assured me that the FL2 sensitivity
>failing will not affect CD4 counts with Simulset.
>
>Any input as to whether the problem is with the PMT or
>the Calibrite beads is welcome. I would like to be
>sure that the problem is with the PMT before
>purchasing it.
>
>Thanking you in advance.
>With warm regards,
>Sandeep.
>
>=====
>Dr. Sandeep Ramalingam,
>Department of Clinical Virology,
>Christian Medical College Hospital,
>Vellore-632004, India.
>Phone(off): 91-416-222102, ext 2070
>Fax: 91-416-232035
>Email: sandeeprk@yahoo.com
>
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>
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:57:30 EST