Hi Everyone, I have recently tried to sort GFP-identified cells from the mouse suprachiasmatic nucleus. Unfortunately, the cells appear to be dead after the FACS sort is completed. I've been using a trypsin dispersion is HBSS (supplemented with Hepes and pen/strep). Does anyone have any ideas of how I can increase cell viability? Thanks in advance! Gilbert -- Gilbert Pitts, Ph.D. Post-doctoral Scholar Dept. of Physiology University of Kentucky 800 Rose St, Room MS508 Lexington, KY 40536-0084 Tel. (859) 257-1645 Fax: (859) 323-1070
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