Dear group, I've been recently having some problems with control antibodies for the intracellular staining. Basically in my tubes with permeabilized monocytes, the control glows in the 2nd log. The same cells, in a non-permeabelized tube, with another reagent for isotype control, ie. surface staining control, is fine (no signal). What I tried doing is using PE-conjuaged anti-CD19 antibody (not found on mono's), which works great as a control - but I'm not sure if this is a good reagent for intracellular staining? Sometimes I read people's materials and methods "irrelevant isotype-matched fluorescent antibody was used.." what exactly does that mean? thanks for any suggestions, maciej ===== `---------------------------------------------` | Maciej S. Simm | 525 E 68th Street | | Research Technician | Room N-805 | | Cornell Medical Center | Tel. 212.746.3428 | `---------------------------------------------` |www.cd4cd8.com | 917-734-6280 | AIM - XsimmX | `---------------------------------------------` __________________________________________________ Do You Yahoo!? Get email at your own domain with Yahoo! Mail. http://personal.mail.yahoo.com/
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