The paper that Howard suggests is: H. A. Crissman, M. H. Hofland, A. P. Stevenson, M. E. Wilder, and R. A. Tobey, Use of DiO-C5-3 to Improve Hoechst Uptake, Resolution of DNA Content, and Survival of CHO Cells. Exp. Cell Res. 174: 388-396 (1988). What was surprising was the effect of DiO-C5-3, particularly on cells that probably have high levels of p-glycoprotein. At 5:06 PM -0500 3/15/01, Howard Shapiro wrote: >Joseph Webster and Mark Kukuruga asked about the viability of cells stained >with Hoechst 33342 for DNA content and sorted after measurement in a UV >illuminating beam. There was an old publication from Los Alamos which, if >I remember correctly, concluded that CHO cell viability was decreased >substantially at laser powers above 100 mW; this presumably involves >interaction of the UV light with the DNA-bound dye. There is much older >literature showing that acridine-stained cells can be killed by blue light, >and that cells stained with methylene blue (which does bind to DNA) can be >killed with red light. The same cells are not harmed substantially by >brief exposure to the dyes if they are not also exposed to light. > >Since DAPI does not normally get into viable eukaryotic cells, there should >not be much reason to worry about effects on the nonstaining viable cells >when DAPI is used as a "nonviability" indicator. > >-Howard
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