Rana, You may want to check out "Three-Color Flow Cytometry Detection of Intracellular Cytokines in Peripheral Blood Mononuclear Cells: Comparative Analysis of Phorbal Myristate Acetate-Ionomycin and Phytohemagglutinin Stimulation" in Clinical Diagnistic Laboratory Immunology, March 2001, p. 303-313, Vol. 8, No. 2. Margaret >Dear Group, > Hi. I am going to start some experiments with cord blood. Specifically I will >be isolating the mononuclear cells from cord blood and use it for expression >studies. These will be activated by PMA/A23187. I would be grateful for any >suggestions and hints for procedures and pitfalls regarding the use of >mononuclear cells from cord blood. Also what are the differences in the >lymphocytes isolated form the pheripheral blood and cord blood (more naive >cell? etc). Thanks a TON in advance for any suggestions (references) and am in >urgent need for feedback. > Rana Nagarkatti (SRF) > Molecular Immunology Lab > Center for Biochemical Technology > Mall Road, Delhi 110007, INDIA. > >____________________________________________________________________ >Get free email and a permanent address at http://www.netaddress.com/?N=1 >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>> Margaret M. Tropea Critical Care Medicine Department National Institutes of Health (ph) 301-496-7752 (fax) 301-480-3389 >>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>>
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