Marcus and Group,
The PMT volt number, is the measurement of the actual voltage applied to
the PMT. Depending upon the configuration of the PMT, this may be a
positive or a negative voltage. If the voltage is applied to the
photocathode (e.g. BD Benchtops) then it will be negative (with respect to
the anode), repelling the photons converted to electrons, to the anode
(output). If the voltage is applied to the anode, it will be positive (with
respect to the photocathode) attracting the electrons to the output. The
operating range of most flow cytometery PMT's is in the 300 to 1000V range.
Hope this helps.
Regards,
Raymond Lannigan
Tritech Inc.
2014 Renard Ct. Suite I
Annapolis, MD 21401
1-410-266-1522
1-800-886-7004
www.tritechinc.com
-----Original Message-----
From: Marcus Reckermann <recker@ftz-west.uni-kiel.de>
To: cyto-inbox
Date: Thursday, March 08, 2001 3:34 PM
Subject: PMT Voltages
>
>Dear flow group,
>
>in my previous mail I asked about the PMT voltages/VOLT units. I mistakenly
>used the threshold as an example, which is of course set as channel number.
>Sorry for this misunderstanding on my behalf.
>
>But I am not sure about for the PMT values. Are these real VOLT units, or
>arbitrariy units on a scale of 1-1000 (e.g. on a BD Vantage)?
>
>marcus
>
>___________________________________________________________
>Marcus Reckermann
>Forschungs- und Technologiezentrum Westküste der Universität Kiel (FTZ)
>(Research and Technology Centre Westcoast of Kiel University)
>Hafentoern
>D-25761 Buesum
>Tel. +49 (0)4834-604-204 or -261
>Fax. +49 (0)4834-604-299
>E-Mail: recker@ftz-west.uni-kiel.de
>FTZ Web Site: www.uni-kiel.de/ftzwest/
>FTZ Flow Cytometry: www.uni-kiel.de/ftzwest/flow/flowhome.htm
>________________________________________________
>Keep your computer busy with www.setiathome.ssl.berkeley.edu
>
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