Hi FLOWers,

From: Heather Medbury (Heather_Medbury@wsahs.nsw.gov.au)
Date: Sun Mar 11 2001 - 17:49:39 EST

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Hi FLOWers,

In your opinion what is the best serum free media to use for culturing lymphocytes
in. I will be doing intracellular cytokine staining of antigen stimulated lymphocytes
but want to avoid using serum.

Any comments on intracellular cytokine staining methods is also welcome.

Heather


Heather Medbury (PhD)
Department of Surgery
Westmead Hospital
Westmead 2145
61-2 9845 7677
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     All experiments are preliminary

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  "In his heart a man plans his course
    but the Lord determines his steps"

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