followup to MetroFlow meeting

From: Maciej Simm (simmmmer@yahoo.com)
Date: Fri Mar 09 2001 - 18:31:59 EST


as usual, it was spectacular, my thanks to the speakers, organizers
and sponsors.

I wonder if anyone could answer my question regarding Dr. Kaplan's
talk about EAS (I'm not sure if he subscribes to this group?).

In the slide showing CD5 and LMP-1(?) expression on B cells as they
were transfected with EBV, the control for CD5 staining gradually
moved towards the right, eventually surpassing 1st log (that's what
it looked like anyway). However the control for LMP-1(?) remained
fixed in every time point.

Is this an artifact due to transformation of the B cells or something
associated with the method itself? And why is there no difference in
the control in the LMP-1 staining over time?

anyway I couldn't figure that out so I wonder if someone with more
experience would take a stab at it....

regards,

Maciej

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