On Tue, 06 Mar 2001 14:59:39 -0500, Richard K. Meister wrote: >We're doing some research in a cat model system and I have heard that the >HNK-1 clone (anti-human CD57) cross-reacts with cat CD57. According to >Linscott's, there is an HNK-1 clone available from BD and Caltag - but >their on-line catalogs don't list it. I'm beginning to suspect that HNK-1 >may not actually be a clone, but rather a "pre-CD57" terminology. Do any >of you know of a clone named HNK-1 and where it can be purchased? HNK-1 is the clone's name which has been assigned to CD57 by the 4th WS; the "brand name" given by BD is Leu-7 (and I'd be surprised if it wasn't in their catalogue any more). >There are several other anti-human CD57 clones that are available. Is it >reasonable to expect that if one CD57 clone cross-reacts with cat CD57 that >all CD57 clones would cross-react, or would each clone need to be tested? >(I'm guessing that each would need to be tested.) yes, even if cumbersome - it needs to be verified in each case. you can find all CD57 that were ever assigned during a Workshop at: http://www.mh-hannover.de/aktuelles/projekte/hlda7/hldabase/CD57.htm there have been two more anti-human clones reported to crossreact with cat CD57: TB01 & NK-1 you may find the reference and the complete x-reactivity table at http://www.mh-hannover.de/aktuelles/projekte/hlda7/animals/ The table also incorporates updated information taken from Pharmingen's poster at HLDA7. >Also, all of the CD57 clones that I've found are IgM! And few are availble >in any form other than purified or FITC-conjugate. Is there an reason why >they would all be IgM isotype? it's a carbohydrate epitope HTH Martin Hadam Martin R. Hadam Kinderklinik - Medizinische Hochschule D-30623 Hannover Germany Email: Hadam.Martin@MH-Hannover.de
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