What is the problem? We did a study on this a couple of years back so maybe I can advise (Barclay, G. R., Walker, B., Gibson, J., McColl, K., and Turner, M. L. (2000) Quality assurance by a commercial flow cytometry method of leucodepletion of whole blood donations: initial application of universal testing and proposals for a batch-release sampling plan. Transfus Med 10: 37-48.). Robin Barclay -----Original Message----- From: MSchwa3722@aol.com <MSchwa3722@aol.com> To: cyto-inbox Date: Wednesday, February 28, 2001 5:07 PM Subject: controls for leucocount procedure > >I am currently working on a flow cytometric procedure for counting residual WBC >in leuko-reduced blood bank products using Beckton-Dickinson's Leucocount Kit. The >FDA mandated control material is normal assayed blood diluted in leucoreduced whole >blood. I have had trouble with the high dilution (1:1000) required to make the control. >I'm unable to get reproducibility. Two commercial companies have also had problems >making an FDA approvable product. Anyone out there perform this test and have any >advice on obtaining acceptable controls??
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