We've been using DAPI for dead cell exclusion for at least the last five years on a Vantage, and for the last year or more on the LSR. It is absolutely identical to PI in terms of the specificity of staining/non-staining. For viability, we use DAPI at 100 nanograms/ml when using cells at ~5 million/ml. If you go up in cell density, you will probably need a higher concentration of DAPI, depending on how many dead cells you have. Go for it. >Hi all, > We are currently evaluating a B-D LSR in order to decide >whether it meets our requirements. One possible configuration we are >considering would use DAPI excited by the UV laser (325nm) for >live-dead discrimination in place of PI. I know we don't need to >devote a channel to PI but some users would prefer to have a separate >live/dead channel and in most experiment we wouldn't be using the UV >laser for anything else. > >My question is has anyone used DAPI for live-dead discrimination? How >does it compare to PI? Are there any particular issues we need to be >aware of? > > >Thanks, > >Adrian Smith -- Howard T. Petrie, Ph.D. Head, Laboratory of Developmental Immunology Memorial Sloan-Kettering Cancer Center Box 341, 1275 York Avenue New York, NY 10021 phone (212)639-2149 fax (212)794-4019
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