BIll, there was a whole discussion on this on this serve back in October, initiated by me for a client of mine.. I received several replies that you should be able to find on the archive. SOrry, I just don't have time to put them all toghether for you. My client was moderately successful at maintaining her GFP during fixation. Candace Enockson --On Tue, Feb 13, 2001 6:56 PM -0800 "Hyun, Bill" <hyun@cc.ucsf.edu> wrote: > > > Hello, > I would greatly appreciate a fixation protocol or reference for running > EGFP and measuring cell cycle with PI for flow. Any help will get a free > beer from Julie Auger. Thanks. > > -Bill > > ***************************** > William C. Hyun, Director > Laboratory for Cell Analysis, S129 > UCSF Comprehensive Cancer Center > San Francisco, CA 94143-0808 > > phone: (415)476-2632 > FAX: (415)476-8218 > email: hyun@cc.ucsf.edu > > Deliveries: > 2340 Sutter Street Rm129 > San Francisco, CA 94115
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