Hallo flowers, We are working with human vascular endothelial cells (HUVEC's) obtained from umbilical cords. We have difficulties in inducing apoptosis in endothelial cells (EC's), besides by irradiation ? We measure apoptosis with FITC-annexin V. Possibly EC's do not exhibit strong annexin V-positivity and show high levels of autofluorescence ? Has anybody experience and can give us a good advice ? We very much appreciate any suggestion or experience. Thanks in advance, yours, Felix Haanen
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