For purposes of interpreting clinical specimens for patient care, I generally consider both populations 1 and 2 to be hematogones, even though some refer to population 2 as intermediate cells. Check references Am J Clin Pathol 114(1): 66, 2000 and Blood 73(2): 543, 1989. Tim Singleton, MD William Beaumont Hospital Royal Oak, MI >>> "Andrea Illingworth" <dcdsflow@mint.net> 02/05/01 01:12PM >>> Another question for the flow experts: When looking at normal B- cells in the bone marrow, one can sometimes differentiate 3 different populations in the bone marrow, representing the various maturational stages of B-cell differentiation: Pop#1: CD19+, CD10+, CD34+, no k/l. CD45 dim, very low FALS Pop#2: CD19+, CD10+ (a little weaker than the first pop.), CD34-, weak CD20 and CD22, no k/l, CD45 ( a little brighter than pop 1), very low FALS Pop#3: CD19+, CD20+ (bright), CD22+ (bright), polyclonal kappa and lambda, bright CD45+ The third population obviously consists of mature B-cells and the first 2 populations are immature B-cell precursors. Which ones are the hematogones, only the first population or is the second population (CD10+,CD34-) also called hematogones? What is the definition of hematogones? Are they always CD34+? We were also wondering why there is no apparent spectrum betwen population 1 and 2, they seem to "jump" from being CD34+ to being CD34 negative without the stages in between. Thank you for any insight Andrea Illingworth DCDS Flow Cytometry Bangor, Maine
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