Hello fellow flowers, Does anybody out there have any special tips/tricks on how to isolate PBMNCs from rabbit blood. I've tried lymphopreping through NycoPrep 1.077A, spinning @ 600xg for 20 mins and didn't get a interface...collected into ACD diluted 1 in 2 in 0.9% NaCl.. The blood was bought in hence was >24 hr old...does the blood need to be freshly taken..? Thanks in advance, Louise Dr. Louise Affleck Senior Scientist Protein Function Group AdProTech Ltd Unit 3, 2 Orchard Road Royston Herts SG8 5HD Tel: +44 (01763) 226090 Fax: +44 (01763) 226021 http://www.adprotech.co.uk
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