Hi Chris and CJ, Yes , it's possible to high-speed sort cells on glass slides. I can tell you that lymphocytes and bacteria don't blow up or anything on the slide- regarding viability on a glass slide, I don't have that data but I would think this isn't a problem since the cells do quite well cloned into microtiter plates. Also, we've seen almost perfect results high-speed sorting E.Coli for clones into solid agar Omniplates. I would think you'd want to prep the slide somehow if you needed viable cells, mainly so the droplet doesn't dry out, but also to contain the sorted drop so it doesn't blend into it's neighboring drop. Possibly try a multiple droplet sort to keep things wet. We did a poster at the last ISAC showing high density sorting of an 8x12 matrix onto a glass slide in the space occupied by a coverslip using the MoFlo. See Cytometry 2000 Supplement 10, page 187 - "Precision X-Y table translation allows single particle sorting into high-density arrays" Peter Lopez Aaron Diamond AIDS Research Center Core Facilities Manager "Chris Worth" <caw@bcc.louisville.edu> on 01/23/2001 12:15:46 PM Please respond to "Chris Worth" <caw@bcc.louisville.edu> To: cyto-inbox cc: Subject: Re: High speed sorting onto a glass slide I would expect the cells to go splat. On Mon, 22 Jan 2001 15:09:51 -0500, CJett wrote: > >One of my colleagues here was asking me if it was possible to high speed >sort onto a glass slide. I've sorted into microtitre plates but never tried >slides....anyone tried this? > >if so how bad was the charge build up from the stream? >were the cells still viable or do they go SPLAT? > >Cj Jett >Scientist: Biomedical Development >Compucyte Corp. >(617)-577-3811 >12 Emily St. >Cambridge Ma 02139-4507 >
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:57:19 EST