Fred, The Litton company has used malaria parasites (P. berghei) as a positive control for the Mouse Micronucleus test (Mutation Research 464 (2000) 195-200), They used PI to define the infected cells. They did not do cell cycle, but I think it would be easy enough to do once the cells are properly gated. Carol W. Johnson DVM PhD Pharmacia Kalamazoo, MI USA "Fred A. Menendez" <fmenende@jhsph.edu> on 01/19/2001 11:59:15 AM To: cyto-inbox cc: (bcc: Carol W Johnson/USKZO/PNU) Subject: Flow Analysis of Plasmodium Compatriots from the Land of Flow: A researcher at our institution wants to use flow cytometry to measure the infection rate of RBCs with malarial parasites by measuring the malarial DNA content of the RBCs. He would use PI and possibly AO as the fluorochromes. He also would like to differentiate at what phase of the cell cycle the majority of the cells are in (?) by determining the number of cells in G0/G1 vs G2/M. While in theory this sounds feasible, and while I have done just such measurements on tissue and WBCs, I have no experience with the measurement of parasitic or RBC nucleic acids. Of course, with the exception of reticulocytes (which can be measured by flow using AO) the vast majority of circulating RBCs contain no nucleic acid. As such, I have a number of technical question I need to answer before we begin these experiments. Any thoughts, ideas, help, or references would be greatly appreciated. Thank you in advance, Fred Menendez Johns Hopkins School of Hygiene and Public Health Flow Cytometry Core Laboratory
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